APR 29, 2021 10:00 AM PDT

Quantitative western blotting analysis: tips, considerations, and streamlining analysis using Invitrogen iBright Analysis Software

Sponsored by: Thermo Fisher Scientific
Speaker
  • Staff Scientist, Thermo Fisher Scientific
    Biography

      Janaki Narahari is a Staff Scientist in the Biosciences Division at Thermo Fisher Scientific. She has broad research experience and has developed several products in the areas of cell and protein biology including western blot imaging and quantification systems. She holds a Ph.D. in biochemistry and has post-doctoral experience in the regulation of viral replication by host cell proteins.


    Abstract

    Date:  April 29, 2021

    Time: 10:00am PDT

     

    Western blotting is a fundamental application used in life science research to better understand target proteins and their function in complex systems. Performing accurate quantitative data analysis from a western blot has been an ongoing challenge due to numerous factors that can introduce variability and affect results. Multiple products and solutions have been introduced that are designed to reduce this variability, improving data reproducibility and subsequent data analysis. In this webinar, we will highlight key considerations for achieving high quality quantitative western blotting including a demonstration on quantitative data analysis using the Invitrogen™ iBright™ Analysis Software. The Invitrogen iBright Analysis Software provides a variety of options and workflow support to improve and facilitate quantitative data analysis.

     

    Learning Objectives

    • Gain insights to best practices and considerations that can improve the quality of western blots and subsequent quantitative data analysis
    • Learn how using optimized normalization techniques, including the novel Invitrogen™ No-Stain™ Protein Labeling Reagent, can adjust for the variability seen in western blotting
    • Demonstrate quantitative data analysis workflows using the iBright Analysis Software

     

    For Research Use Only. Not for use in diagnostic procedures. 2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified

     

    Webinars will be available for unlimited on-demand viewing after live event.


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