OCT 12, 2021 8:00 AM PDT

The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments.

Speaker

Abstract
Date:  October 12, 2021
Time: 8:00am (PDT),  11:00am (EDT)
 

CRISPR-Cas9 is a nuclease-based genome editing system which has seen exponential growth in adoption with broad applications from basic research to therapeutics.

CRISPR mediated genome editing is an extremely powerful tool capable of achieving high editing efficiencies in wide range of different cell types and gene loci. However, this high editing efficiency comes at a price: off target cleavage. These unintended “off target” edits to the genome can result in undesired phenotypes and slow the progression of your project.

In this presentation, we will discuss some common causes of off-target effects during genome editing, as well as explore some strategies that can be taken to help minimize the occurrence of these off-target events during CRISPR editing in iPSCs and T-cells.

 
Learning Objectives
  • Decribe the impact of off-target events in CRISPR-Cas9 editing
  • Discuss methods to detect & identify off-target events
  • Discuss strategies to help minimize off-target events during high fidelity Cas9 variants and optimizing gRNA design
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
LabRoots is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E. ® Program. By attending this webinar, you can earn 1 Continuing Education credit once you have viewed the webinar in its entirety.

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