SEP 20, 2022 9:00 AM PDT

Breaking through RT-qPCR boundaries in gene expression analysis

Sponsored by: Thermo Fisher Scientific
Speaker

Abstract
First Broadcast:
Date:  September 20, 2022
Time: 9:00am PDT, 12:00pm EDT, 6:00pm CEST
 
Second Broadcast:
Date: September 21, 2022
Time: 9:00am SGT
 
 
Variation in gene expression among cell types, tissues, and organisms is commonly examined by reverse transcription quantitative PCR, or RT-qPCR. In this process, RNA is isolated from samples of interest and reverse-transcribed into complementary DNA (cDNA). The levels of gene expression are then determined from the quantity of cDNA amplified by PCR. The initial stages of the RT-qPCR workflow, such as RNA isolation and reverse transcription, can critically impact the accuracy of the quantitation of gene expression.
 
Learning Objectives:
  • Important considerations for RNA isolation
  • Tools for efficient cDNA preparation
  • Sources of poor RT-qPCR results
  • Tips for overcoming challenges, for highest success
 
 
Webinars will be available for unlimited on-demand viewing after live event.
 
 

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